A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction
نویسندگان
چکیده
منابع مشابه
A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction.
BACKGROUND The measurement of C-reactive protein (CRP) concentrations has been of interest as a classical marker of acute phase response; in addition, it has been of particular interest in cardiovascular risk stratification where high-sensitive measurements are necessary. Since CRP is able to bind phospholipids (mainly phosphocholine) in the presence of calcium ions, we explored the possibiliti...
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BACKGROUND C-reactive protein (CRP) is able to bind phospholipids (mainly phosphocholine) in the presence of calcium ions. We investigated the use of this property for developing an affordable turbidimetrical CRP assay based on diluted soy oil. METHODS Serum (or heparinized plasma) was mixed with Intralipid 20% in Tris-calcium buffer (pH 7.5). After 30 min of incubation at 37 degrees C, the C...
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C-reactive protein (CRP) is able to bind phosphocholine in the presence of calcium ions. According to a previous functional property of CRP, we tried to develop an affordable and cheap high sensitive nephelometric CRP assay using soy oil. Serum samples were measured by Nephelometer BNII (Siemens), by mixing the serum with diluted soy oil emulsion (Intralipid ® 20%) and Tris-calcium buffer (PH 7...
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Background and Objective: The aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-CRP) in preeclampsia and to compare hs-CRP levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. Materials and Methods: Serum hs-CRP levels were inv...
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background and objective: the aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-crp) in preeclampsia and to compare hs-crp levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. materials and methods: serum hs-crp levels were investigated in 40 cases of normal pregnant women, 37 cases with mild preeclampsia and 38 cases...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine (CCLM)
سال: 2014
ISSN: 1437-4331,1434-6621
DOI: 10.1515/cclm-2013-0669